Global population growth is projected to outpace plant-breeding improvements in major crop yields within decades. To ensure future food security, multiple creative efforts seek to overcome limitations to crop yield. Perhaps the greatest limitation to increased crop yield is photosynthetic inefficiency, particularly in C3 crop plants. Recently, great strides have been made toward crop improvement by researchers seeking to introduce the cyanobacterial CO2-concentrating mechanism (CCM) into plant chloroplasts. This strategy recognises the C3 chloroplast as lacking a CCM, and being a primordial cyanobacterium at its essence. Hence the collection of solute transporters, enzymes, and physical structures that make cyanobacterial CO2-fixation so efficient are viewed as a natural source of genetic material for C3 chloroplast improvement. Also we highlight recent outstanding research aimed toward the goal of introducing a cyanobacterial CCM into C3 chloroplasts and consider future research directions.
Highlights
• Improvement of photosynthesis in C3 crop plants is needed to secure future yields.
• Cyanobacterial CO2-concentrating mechanisms are a potential source to exploit.
• Components of these mechanisms include transporters and RuBisCO micro-compartments.
• Advances include expression of transporters and carboxysome components in plants.
• Current research foci are transporter locations, activation, and RuBisCO folding.
In photosynthesis Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalyses the often rate limiting CO2-fixation step in the Calvin cycle. This makes Rubisco both the gatekeeper for carbon entry into the biosphere and a target for functional improvement to enhance photosynthesis and plant growth. Encumbering the catalytic performance of Rubisco is its highly conserved, complex catalytic chemistry. Accordingly, traditional efforts to enhance Rubisco catalysis using protracted “trial and error” protein engineering approaches have met with limited success. Here we demonstrate the versatility of high throughput directed (laboratory) protein evolution for improving the carboxylation properties of a non-photosynthetic Rubisco from the archaea Methanococcoides burtonii. Using chloroplast transformation in the model plant Nicotiana tabacum (tobacco) we confirm the improved forms of M. burtonii Rubisco increased photosynthesis and growth relative to tobacco controls producing wild-type M. burtonii Rubisco. Our findings indicate continued directed evolution of archaeal Rubisco offers new potential for enhancing leaf photosynthesis and plant growth.
The temperature responses of mesophyll conductance (gm) were investigated for nine species using carbon isotope techniques combining tunable diode laser spectroscopy and gas exchange measurements. Species included the evergreen trees Eucalyptus pauciflora and Quercus engelmannii; the tropical evergreen tree Lophostemon confertus; as well as the herbaceous species Nicotiana tabacum, Oryza sativa, Triticum aestivum, Gossypium hirsutum, Glycine max and Arabidopsis thaliana. Responses varied from a two- to threefold increase in mesophyll conductance between 15 and 40 °C observed for N. tabacum, G. hirsutum, G. max and E. pauciflora to almost no change in L. confertus and T. aestivum. To account for the different temperature responses between species, we suggest that there must be variation in both the activation energy for membrane permeability and the effective pathlength for liquid phase diffusion. Stomatal conductance was relatively independent of increases in leaf temperature and concomitant increases in leaf to air vapour pressure difference. Two exceptions were Eucalyptus and Gossypium, where stomatal conductance increased with temperature up to 35 °C despite increasing leaf to air vapour pressure. For a given species, temperature responses of stomatal and mesophyll conductance were independent of one another.
